The National Theatre (Czech: Národní divadlo) is a historic opera house in Prague, Czech Republic. It is known as the alma mater of Czech opera, and as the national monument of Czech history and art.

The National Theatre belongs to the most important Czech cultural institutions, with a rich artistic tradition, which helped to preserve and develop the most important features of the nation–the Czech language and a sense for a Czech musical and dramatic way of thinking.

Today the National Theatre consists of three artistic ensembles: opera, ballet and drama. They alternate in their performances in the historic building of the National Theatre, in the State Opera, in the Estates Theatre and in the Kolowrat Theatre. All three artistic ensembles select their repertoire both from classical heritage, and modern authors.